FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ada2b, Sgf11
FlyBase ID
FBig0000102204
Interaction Network
Interactions Browser links
Ada2b network
Sgf11 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204567-5.coIP.WB
Reference
Weake et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sgf11 

Sgf11

bait

HA tag

Ada2b 

dAda2b

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Weake et al., 2008)
FBrf0209543-15.coIP.MS
Reference
Weake et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2B

bait

FLAG tag, HA tag

Sgf11 

Sgf11

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Weake et al., 2009)

The Drosophila SAGA complex is composed of the following subunits: Gcn5, Ada2b, dik, Sgf29, CG6506, Ada1-2, Spt3, Spt20, Nipped-A, e(y)1 (Taf9), Taf10b, Taf12, wda, Saf6, not, Sgf11, e(y)2 and Atxn7.

(Weake et al., 2009)

Tandem affinity purification of bait and identification of prey by MudPIT.

(Weake et al., 2009)
FBrf0214372-10.coIP.MS
Reference
Weake et al., 2011
Description
physical association
Assay
Collection
Source/Stage
larval muscle system / embryonic stage 10embryonic stage 17late extended germ band stageembryonic stage 13embryonic stage 11embryonic stage 12embryonic stage 14embryonic stage 15dorsal closure stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2b

bait

FLAG tag

Sgf11 

Sgf11

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in muscle (Mef2-GAL4); prey produced from endogenous gene.

(Weake et al., 2011)

All SAGA subunits were present in purifications from both muscle and neuronal cells.

(Weake et al., 2011)

Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.

(Weake et al., 2011)
FBrf0214372-27.coIP.MS
Reference
Weake et al., 2011
Description
physical association
Assay
Collection
Source/Stage
larval nervous system / embryonic stage 10embryonic stage 17late extended germ band stageembryonic stage 13embryonic stage 11embryonic stage 12embryonic stage 14embryonic stage 15dorsal closure stageembryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ada2b 

Ada2b

bait

HA tag, FLAG tag

Sgf11 

Sgf11

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct in nervous system (elav-GAL4); prey produced from endogenous gene.

(Weake et al., 2011)

All SAGA subunits were present in purifications from both muscle and neuronal cells.

(Weake et al., 2011)

Proteins that non-specifically bind FLAG beads were identified in control purifications from untagged extract (OregonR) and excluded from further analysis.

(Weake et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)