FB2025_02 , released April 17, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
spag, rept
FlyBase ID
FBig0000102470
Interaction Network
Interactions Browser links
spag network
rept network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0224244-5.coIP.MS
Reference
Benbahouche et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
spag 

Spag

bait

FLAG tag

spag 
rept 

CG9750

prey

3 peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spag 
TPR motifs
necessary binding region
aa 96-197
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was soluble cytosolic extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Benbahouche et al., 2014)

Affinity purification of bait and identification of prey by MALDI peptide mapping.

(Benbahouche et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)