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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000102604
Interaction Network
Interactions Browser links
vas network
faf network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0167410-1.coIP.XL.MS
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 
VAS
bait
faf 
FAF
prey
45 unique peptides identified
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
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Isoform-specific participants
Corresponds to
Description
Role
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Comments concerning this interaction
Source was cross-linked embryonic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
In a faf mutant background, vas protein levels are reduced to 51% and 12% of wild-type levels in ovaries and embryos, respectively; polyubiquitinated vas is detected.
Embryonic extracts were cross-linked before the first vas immunoprecipitation. Complexes were isolated by "continuous-elution electrophoresis", then vas was immunoprecipitated again. Purified complexes were subjected to mass spectrometry for protein identification.
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)