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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000102604
Interaction Network
Interactions Browser links
vas network
faf network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0167410-1.coIP.XL.MS
Reference
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vas 

VAS

bait
faf 

FAF

prey

45 unique peptides identified

Experimental entities
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Identifier
Reported
Role
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Subregions with role in interaction
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Description
Role
Coordinates
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Isoform-specific participants
Corresponds to
Description
Role
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Comments concerning this interaction

Source was cross-linked embryonic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

In a faf mutant background, vas protein levels are reduced to 51% and 12% of wild-type levels in ovaries and embryos, respectively; polyubiquitinated vas is detected.

Embryonic extracts were cross-linked before the first vas immunoprecipitation. Complexes were isolated by "continuous-elution electrophoresis", then vas was immunoprecipitated again. Purified complexes were subjected to mass spectrometry for protein identification.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)