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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000102658
Interaction Network
Interactions Browser links
Arf102F network
rb network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0217370-21.PD.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Arf4

bait

His tag

rb 

AP-3β

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

rb interacts with GTP-bound forms of both Arf79f and Arf102f.

The N-terminal amphipathic helix of Arf protein was replaced with 10-His residues. His-tagged bait Arf protein was then used to coat the surface of liposomes containing low levels of a lipid with Ni-nitrilotriacetic acid (NTA) head group. Bait-coated liposomes are mixed with cell lysate, then liposomes are purified by centrifugation. This allows for the identification of protein interactions that occur preferentially or exclusively at the surface of a lipid bilayer.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)