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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000102926
Interaction Network
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ProtA network
Nlp network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0226246-3.coIP.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Mst35Ba

bait

V5 tag

Nlp 

NLP

prey

6 peptide matches, 31% coverage

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

Recombinant purified ProtA and ProtB were mixed with DNA to generate "model sperm chromatin", then incubated with an embryonic extract capable of evicting protamines from DNA. This mixture was fractionated by sucrose gradient, and fractions containing evicted protamines were subjected to anti-V5 immunoprecipitation of tagged protamine. Purified endogenous proteins were identified by mass spectrometry.

FBrf0234485-18.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlp 

NLP

bait

GST tag

ProtA

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)