Source was adult mitochondrial preparation wild-type fly line; proteins produced from endogenous genes.
Protein complexes were isolated from mitochondria by non-denaturing blue native page. Complex activity was confirmed by in-gel enzymatic staining assays. Bands were excised and subjected to mass spectrometry for protein identification.
Characterization of Drosophila melanogaster mitochondrial cytochrome c oxidase (COX) complex.
Source was larvae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
