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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103107
Interaction Network
Interactions Browser links
Diap1 network
Ubr3 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0206305-6.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIAP1

bait

GTC tag

Ubr3 

UBR3

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain
sufficient binding region
aa 21-438
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0226660-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubr3 

Ubr3

bait

FLAG tag

Ubr3 
Ubr3 

DIAP1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ΔN form
sufficient binding region
aa 21-438

mimics the caspase cleaved form

neoepitope
mutation disrupting interaction
aa 21

N21M

Ubr3 
N-terminal and central regions
sufficient binding region
aa 1-1514

ΔRING construct, coordinates relative to Ubr3-PA

Ubr3 
UBR domain
necessary binding region
aa 222-292

ΔUBR construct, coordinates relative to Ubr3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Ubr3 binds more strongly to N-terminally cleaved Diap1.

FBrf0226660-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIAP1

bait

V5 tag

Ubr3 

Ubr3

prey

FLAG tag

Ubr3 
Ubr3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ΔN form
sufficient binding region
aa 21-438

mimics the caspase cleaved form

neoepitope
mutation disrupting interaction
aa 21

N21M

Ubr3 
N-terminal and central regions
sufficient binding region
aa 1-1514

ΔRING construct, coordinates relative to Ubr3-PA

Ubr3 
UBR domain
necessary binding region
aa 222-292

ΔUBR construct, coordinates relative to Ubr3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Ubr3 binds more strongly to N-terminally cleaved Diap1.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0226660-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIAP1

bait

V5 tag

Ubr3 

Ubr3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0229651-10.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubr3 

Ubr3

bait

GFP tag

DIAP1

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Cells treated with MG132 to stabilize proteins from proteasomal degradation.

FBrf0229651-9.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DIAP1

bait

FLAG tag

Ubr3 

Ubr3

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Cells treated with MG132 to stabilize proteins from proteasomal degradation.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)