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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103371
Interaction Network
Interactions Browser links
run network
gro network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0098186-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
run 

Runt

bait

GST tag

run 
run 
gro 

Groucho

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
run 
C-terminal region
sufficient binding region
aa 461-510

coordinates relative to run-PA, reported as aa 460-509

run 
WRPW motif
necessary binding region
WRPY
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

FBrf0098186-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
run 

Runt

bait

fused to GAL4 DNA-binding domain

run 
run 
run 
gro 

Gro

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
run 
WRPW motif
sufficient binding region
VWRPY
run 
WRPW motif
necessary binding region
WRPY
run 
Runt domain
mutation increasing interaction
aa 510

coordinates relative to run-PA, reported as Y509W

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

The N-terminal "Runt domain" (aa 104-231) of run inhibits binding to gro in the two hybrid assay. Mutation of the C-terminal residue from Y to W bypasses this inhibition.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0099085-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

Gro

bait

fused to LexA DNA-binding domain

run 

Runt

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/VP16-AD

Positive control.

FBrf0213441-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
run 

Runt

bait

fused to LexA DNA-binding domain

run 
gro 

Groucho

prey

fused to VP16 activation domain

gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
WD domain
sufficient binding region
aa 338-719

coordinates relative to gro-PA

run 
VWRPY motif
necessary binding region
aa 485-509
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/VP16-AD

The run bait lacks the "Runt domain" (aa 110-225), which is known to inhibit the run-gro interaction.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)