Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell detergent-free extract (endogenous gene).

Specificity S-score=27.55.

Constitutively active (GTP-locked) form of Rab was used for pull down. Parallel pull downs performed for several Rabs proteins in this study. Yield was measured using spectral counts. A specificity score (S score) was calculated, where greater weight was given to interactions seen with fewer baits.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

The Rab6-Gorab interaction is disrupted in the presence of Sas-6.

Comparison of HDX patterns of Gorab alone and in complex with Rab6 predicts the aa 190-320 segment of Gorab as involved in the interaction.

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

The Rab6-Gorab interaction was measured with a GTP locked mutant of Rab6, Kd = 2.24uM.