Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Treatment of cells with hh conditioned medium or depletionof smo increased the ptc-Smurf interaction, while co-expression of smo decreased the interaction.