FB2019_05, released Oct 15, 2019

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
ptc, Smurf
FlyBase ID
FBig0000103473
Interaction Network
Interactions Browser links
ptc network
Smurf network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0225546-1.Y2H
Reference
Huang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Smurf 
Smurf
bait
fused to GAL4 DNA-binding domain
ptc 
Ptc
prey
fused to GAL4 activation domain
ptc 
ptc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ptc 
C-terminal region L1
sufficient binding region
aa 1198-1286
ptc 
C-terminal domain
mutation disrupting interaction
aa 1206-1209
PPAY to AAAA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).
(Huang et al., 2013)
3 of 47 clones recovered using Smurf as bait represented ptc clones.
(Huang et al., 2013)
All three "C2-WW-HECT" E3 family proteins can bind to the C-terminal domain of ptc : Su(dx), Nedd4 and Smurf.
(Huang et al., 2013)
Two-hybrid system: yeast GAL4-BD/GAL4-AD
(Huang et al., 2013)
FBrf0225546-2.coIP.WB
Reference
Huang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Smurf 
Smurf
bait
FLAG tag
Smurf 
Smurf 
ptc 
Ptc
prey
Myc tag
ptc 
ptc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ptc 
C-terminal domain
sufficient binding region
aa 1101-1286
"CTD"
ptc 
C-terminal domain
necessary binding region
aa 1101-1286
"ΔCTD" construct
Smurf 
WW domain
sufficient binding region
Smurf 
WW domain
necessary binding region
"ΔWW" construct
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
(Huang et al., 2013)
Smurf promotes both Ub K48-linked and Ub K63-linked ubiquitination of ptc.
(Huang et al., 2013)
FBrf0225546-3.coIP.WB
Reference
Huang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ptc 
Ptc
bait
Myc tag
Smurf 
Smurf
prey
FLAG tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
(Huang et al., 2013)
FBrf0225546-4.coIP.WB
Reference
Huang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ptc 
Ptc
bait
Smurf 
Smurf
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
(Huang et al., 2013)
FBrf0225546-5.PD.WB
Reference
Huang et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ptc 
Ptc
bait
GST tag
ptc 
ptc 
Smurf 
Smurf
prey
His tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ptc 
C-terminal domain
sufficient binding region
aa 1101-1286
"CTD"
ptc 
C-terminal domain
mutation disrupting interaction
aa 1206-1209
PPAY to AAAA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
(Huang et al., 2013)
FBrf0238147-4.coIP.WB
Reference
Li et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ptc 
Ptc
bait
Myc tag
Smurf 
Smurf
prey
FLAG tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Treatment of cells with hh conditioned medium or depletionof smo increased the ptc-Smurf interaction, while co-expression of smo decreased the interaction.
(Li et al., 2018)
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
(Li et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)