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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103473
Interaction Network
Interactions Browser links
Smurf network
ptc network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0225546-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Smurf

bait

fused to GAL4 DNA-binding domain

ptc 

Ptc

prey

fused to GAL4 activation domain

ptc 
ptc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ptc 
C-terminal region L1
sufficient binding region
aa 1198-1286
ptc 
C-terminal domain
mutation disrupting interaction
aa 1206-1209

PPAY to AAAA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

3 of 47 clones recovered using Smurf as bait represented ptc clones.

All three "C2-WW-HECT" E3 family proteins can bind to the C-terminal domain of ptc : Su(dx), Nedd4 and Smurf.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0225546-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Smurf

bait

FLAG tag

ptc 

Ptc

prey

Myc tag

ptc 
ptc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ptc 
C-terminal domain
sufficient binding region
aa 1101-1286

"CTD"

ptc 
C-terminal domain
necessary binding region
aa 1101-1286

"ΔCTD" construct

WW domain
sufficient binding region
WW domain
necessary binding region

"ΔWW" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Smurf promotes both Ub K48-linked and Ub K63-linked ubiquitination of ptc.

FBrf0225546-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ptc 

Ptc

bait

Myc tag

Smurf

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0225546-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ptc 

Ptc

bait

Smurf

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0225546-5.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ptc 

Ptc

bait

GST tag

ptc 
ptc 

Smurf

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ptc 
C-terminal domain
sufficient binding region
aa 1101-1286

"CTD"

ptc 
C-terminal domain
mutation disrupting interaction
aa 1206-1209

PPAY to AAAA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0238147-4.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ptc 

Ptc

bait

Myc tag

Smurf

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Treatment of cells with hh conditioned medium or depletionof smo increased the ptc-Smurf interaction, while co-expression of smo decreased the interaction.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)