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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103578
Interaction Network
Interactions Browser links
Rab2 network
CG9590 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0226902-12.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab2 

Rab2

bait

fused to GAL4 DNA-binding domain

Rab2 
Rab2 

CG9590

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab2 
GTPase domain
mutation increasing interaction
aa 65

Q65L, GTP-locked

Rab2 
GTPase domain
mutation disrupting interaction
aa 20

S20N, GDP-locked

N-terminal region
sufficient binding region
aa 1-272
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

CG9590 interacts with GTP-locked Rab2 (and more weakly, with GTP-locked Rab14), but neither form of the other 15 Rabs tested.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0226902-8.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab2 

Rab2

bait

GST tag

Rab2 
Rab2 

CG9590

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab2 
GTPase domain
mutation increasing interaction
aa 65

Q65L, GTP-locked

Rab2 
GTPase domain
mutation disrupting interaction
aa 20

S20N, GDP-locked

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

CG9590 specifically binds the GTP-bound state of Rab2 or Rab14 (not GDP-locked forms).

FBrf0229467-19.PD.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab2 

Rab2

bait

GST tag

CG9590

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell detergent-free extract (endogenous gene).

Specificity S-score=9.59.

Constitutively active (GTP-locked) form of Rab was used for pull down. Parallel pull downs performed for several Rabs proteins in this study. Yield was measured using spectral counts. A specificity score (S score) was calculated, where greater weight was given to interactions seen with fewer baits.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)