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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103582
Interaction Network
Interactions Browser links
Rab6 network
CG8578 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0226902-21.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab6 

Rab6

bait

GST tag

Rab6 
Rab6 

CG8578

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab6 
GTPase domain
mutation increasing interaction
aa 71

Q71L, GTP-locked

Rab6 
GTPase domain
mutation disrupting interaction
aa 26

T26N, GDP-locked

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

CG8578 specifically binds the GTP-bound state of Rab6 (not the GDP-locked form).

FBrf0229467-72.PD.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab6 

Rab6

bait

GST tag

CG8578

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell detergent-free extract (endogenous gene).

Specificity S-score=14.39.

Constitutively active (GTP-locked) form of Rab was used for pull down. Parallel pull downs performed for several Rabs proteins in this study. Yield was measured using spectral counts. A specificity score (S score) was calculated, where greater weight was given to interactions seen with fewer baits.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)