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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103728
Interaction Network
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lola network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0159022-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lola 

Lola

bait

zf5 isoform

lola 

Lola

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; protein produced from endogenous gene.

The lola zf5 (lola-PD) 105 kDa isoform was immunoprecipitated using the isoform-specific 7F1 monoclonal antibody. Other coimmunoprecipitated isoforms of lola (of different molecular weight) were detected using a polyclonal lola antiserum.

FBrf0222788-6.PD.TV
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lola 

LOLA

bait

MBP tag

lola 

LOLA

prey

luciferase tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

From an analysis of many TF-ChIP data sets, TF-TF interactions were predicted on the basis of regular DNA sequence motif arrangements. 27 predicted TF-TF interactions were tested using a pull down assay that measures the \'Luminescence Intensity Ratio\'. A cut-off of 7 was used, well above values for six negative controls.

Homotypic interactions confirmed for lola-PA, lola-PD and lola-PQ isoforms.

Interaction in vitro; bait produced by coupled in vitro translation; prey produced by coupled in vitro translation.

FBrf0234063-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lola 

LOLA

bait

fused to GAL4 DNA-binding domain

lola 

LOLA

prey

fused to GAL4 activation domain

lola 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lola 
BTB domain
sufficient binding region
aa 1-124

coordinates relative to lola-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

External Crossreferences and Linkouts ( 0 )
References (3)