FB2021_01, released Feb 18, 2021

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
galla-2, crb
FlyBase ID
FBig0000103827
Interaction Network
Interactions Browser links
galla-2 network
crb network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0228544-5.coIP.WB
Reference
Yeom et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
crb 

Crb

bait

Myc tag

crb 
galla-2 

Galla-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
intracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Yeom et al., 2015)

Galla-2 shows more efficient interaction with crb when the JM and PBM domains of crb are mutated.

(Yeom et al., 2015)

MBP-Crb[intra] (intracellular domain) served as bait in the experiment.

(Yeom et al., 2015)
FBrf0228544-6.PD.WB
Reference
Yeom et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
galla-2 

Galla-2

bait

GST tag

crb 

Crb

prey

MBP tag

crb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
crb 
intracellular domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Yeom et al., 2015)

MBP-Crb[intra] (intracellular domain) served as prey in the experiment.

(Yeom et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)