Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103854
Interaction Network
Interactions Browser links
run network
H network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0213441-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
run 

Runt

bait

fused to LexA DNA-binding domain

run 
H 

Hairless

prey

fused to VP16 activation domain

H 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
H 
C-terminal region
sufficient binding region
aa 672-1077

coordinates relative to H-PA

run 
VWRPY motif
necessary binding region
aa 485-509
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/VP16-AD

The run bait lacks the "Runt domain" (aa 110-225), which is known to inhibit the run-gro interaction.

FBrf0213441-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
H 

Hairless

bait
run 

Runt

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)