Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000103922
Interaction Network
Interactions Browser links
Chi network
run network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0229654-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chi 

Chip

bait

FLAG tag

run 

Runt

prey

HA tag

run 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
run 
interaction domain
necessary binding region
aa 229-510

229 aa starting position for this fragment is approximate.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction requires C-terminal and aa 229 - 510 (229 is approximate) of run.

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0229654-4.NMR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chi 

Chip

bait
run 

Runt

prey

15[N] label

run 
run 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
run 
interaction domain
sufficient binding region
aa 228-257
run 
NPF motif
mutation disrupting interaction
aa 247-249

NPF to AAA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

run sequence used for the mutation disrupting interaction is a subset of the full amino acid sequence, aa 228-257, with mutated NPF motif, NPF to AAA.

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)