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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000104046
Interaction Network
Interactions Browser links
norpA network
inaD network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0091374-1.FW.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

norpA

bait
inaD 

INAD

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from adult head extract; prey produced and labeled by in vitro translation.

The inaD probe binds a ~130kDa band that is 1) recognized by anti-norpA antibody and 2) absent in norpA mutant background.

Radiolabelled inaD protein was overlayed onto blots containing adult head protein extracts from various genotypes separated by SDS-PAGE.

FBrf0096480-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

inaD

bait

PLC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was membranes from adult heads of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

trp, norpA and inaC, but not ninaE or Gαq, coimmunoprecipitate with inaD.

FBrf0096480-6.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait

GST tag

inaD 

PLC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ5 domain
sufficient binding region
aa 580-665

coordinates relative to inaD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

FBrf0099512-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait

NORPA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0099512-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

NORPA

bait
inaD 

INAD

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0099512-3.FW.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

norpA

bait

GST tag

inaD 

INAD

prey

[35]S label

inaD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
sufficient binding region
aa 1092-1095
C-terminal region residue F1093
mutation disrupting interaction
aa 1093

F1093L or F1093V

C-terminal region residue C1094
mutation disrupting interaction
aa 1094

C1094S or C1094R

inaD 
C-terminal region
sufficient binding region
aa 327-674

coordinates relative to inaD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0102437-1.FW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

norpA

bait

HA tag

inaD 

inaD

prey

GST tag

inaD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ1 domain
sufficient binding region
aa 10-112

coordinates relative to inaD-PA

C-terminus residue A1095
mutation increasing interaction
aa 1095

A1095F

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected COS cell lysate; prey produced as a recombinant fusion protein in bacterial system.

The consensus binding motif for inaD PDZ1 domain is X(F/Y/W)CF, as determined by peptide screening.

norpA was immunoprecipitated from transfected COS cells, separated by SDS-PAGE, transferred to nitrocellulose and incubated with GST-inaD.

FBrf0102437-2.FW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

inaD

bait

GST tag

inaD 

norpA

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ1 domain
sufficient binding region
aa 10-112

coordinates relative to inaD-PA

C-terminus
sufficient binding region
aa 1080-1095
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

GST-inaD was separated by SDS-PAGE, transferred to nitrocellulose and incubated with MBP-norpA.

FBrf0102437-3.ELISA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

norpA

bait

MBP tag

inaD 

inaD

prey

GST tag

inaD 
inaD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ1 domain
sufficient binding region
aa 10-112

coordinates relative to inaD-PA

inaD 
PDZ5 domain
sufficient binding region
aa 580-665

coordinates relative to inaD-PA

C-terminus
sufficient binding region
aa 1080-1095
G Box Homology region
sufficient binding region
aa 972-1070
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Bipartite binding of inaD and norpA. The inaD PDZ1 domain binds the norpA C-terminus, and the inaD PDZ5 domain binds the norpA G box homology region.

FBrf0102437-4.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

inaD

bait

GST tag

inaD 
inaD 

norpA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ1 domain
sufficient binding region
aa 10-112

coordinates relative to inaD-PA

inaD 
PDZ5 domain
sufficient binding region
aa 580-665

coordinates relative to inaD-PA

C-terminal region
necessary binding region
aa 973-1095
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type or transgenic adult head extract.

FBrf0102437-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

inaD

bait

norpA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
necessary binding region
aa 973-1095
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type or transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0103542-10.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

PLC

bait

Myc tag

inaD 

INAD

prey

FLAG tag

inaD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ1 domain
sufficient binding region
aa 1-140

coordinates relative to inaD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0132378-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait

PLC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult head membrane extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Using heat shock constructs in an inaD mutant background, the authors show that inaD interacts with norpA and inaC shortly after heat shock induced expression (3hr), long before these proteins reach the rhabdomeres and long before light

responses have recovered. This suggests that the complex is preassembled before transport.

FBrf0137228-1.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

inaD

neutral component
inaD 

inaD

neutral component
inaD 
inaD 

inaD

neutral component
inaD 

norpA

neutral component

norpA

neutral component

norpA

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ1 domain
sufficient binding region
aa 11-107

coordinates relative to inaD-PA

C-terminus
sufficient binding region
aa 1089-1095
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; peptide chemically synthesized.

FBrf0137228-2.CH.CS.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

inaD

neutral component
inaD 

inaD

neutral component
inaD 
inaD 

inaD

neutral component
inaD 

norpA

neutral component

norpA

neutral component

norpA

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ1 domain
sufficient binding region
aa 11-107

coordinates relative to inaD-PA

C-terminus
sufficient binding region
aa 1089-1095
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

The inaD PDZ1 domain forms a disulfide bridge with the norpA C-terminus, as evidenced by the fact that the complex persists during SDS-PAGE, but is disrupted by reducing agents.

Proteins were fractionated by size exclusion and complexes analyzed by SDS-PAGE in the presence or absence of reducing agents.

FBrf0241103-1.ITC
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

unspecified role
inaD 

INAD

unspecified role
inaD 
inaD 
inaD 
inaD 
inaD 
inaD 

INAD

unspecified role
inaD 
inaD 
inaD 
inaD 
inaD 

NORPA

unspecified role

NORPA

unspecified role

NORPA

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ45 domain
sufficient binding region
aa 473-674

coordinates relative to inaD-PA

CC,PBM domain
sufficient binding region
aa 863-1095

coordinates relative to norpA-PA

PBM domain
necessary binding region
aa 1093-1095

coordinates relative to norpA-PA

PBM domain residue 1093
mutation disrupting interaction
aa 1093

F1093A, coordinates relative to norpA-PA

PBM domain residue 1095
mutation decreasing interaction
aa 1095

A1095L, coordinates relative to norpA-PA

PBM domain residue 1094
mutation decreasing interaction
aa 1094

Y1094A, coordinates relative to norpA-PA

PBM domain residue 1092
mutation decreasing interaction
aa 1092

E1092A, coordinates relative to norpA-PA

coiled coil domain residue 1081
mutation decreasing interaction
aa 1081

E1081A, coordinates relative to norpA-PA

coiled coil domain residue 1085
mutation decreasing interaction
aa 1085

Y1085E, coordinates relative to norpA-PA

coiled coil domain residue 952
mutation decreasing interaction
aa 952

R952A, coordinates relative to norpA-PA

coiled coil domain residue 944
mutation decreasing interaction
aa 944

W944Q, coordinates relative to norpA-PA

inaD 
PDZ4 domain residues 557,561
mutation decreasing interaction
aa 557,561

H557A,H561A, coordinates relative to inaD-PA

inaD 
PDZ4 domain residues 521
mutation decreasing interaction
aa 521

Y521A, coordinates relative to inaD-PA

inaD 
PDZ5 domain residues 642
mutation decreasing interaction
aa 642

F642A, coordinates relative to inaD-PA

inaD 
PDZ5 domain residues 646
mutation decreasing interaction
aa 646

Y646A, coordinates relative to inaD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The strength of the norpA-inaD interaction decreases with mild acidifcation.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0241103-2.FPS
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait
inaD 

NORPA

prey

FITC label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PBM domain
sufficient binding region
aa 1086-1095

coordinates relative to norpA-PA

inaD 
PDZ45 domain
sufficient binding region
aa 473-674

coordinates relative to inaD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; labeled prey peptide commercially synthesized.

FBrf0241103-3.XC
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

neutral component
inaD 

INAD

neutral component
inaD 
inaD 

INAD

neutral component
inaD 

NORPA

neutral component

NORPA

neutral component

NORPA

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ45 domain
sufficient binding region
aa 473-674

coordinates relative to inaD-PA

CC,PBM domain
sufficient binding region
aa 863-1095

coordinates relative to norpA-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

A mutant form of norpA in which 8 lysines are mutated to alanine (K880A,K884A,K887A,K888A,K891A,K898A,K899A,K902A) was used to determine the crystal structure.

FBrf0241103-4.NMR
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

neutral component
inaD 

INAD

neutral component
inaD 
inaD 

INAD

neutral component
inaD 

NORPA

neutral component

NORPA

neutral component

NORPA

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ45 domain
sufficient binding region
aa 473-674

coordinates relative to inaD-PA

CC,PBM domain
sufficient binding region
aa 863-1095

coordinates relative to norpA-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0241103-5.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

NORPA

bait

StrepII tag

inaD 

INAD

prey

GFP tag

inaD 
inaD 
inaD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ45 domain
sufficient binding region
aa 473-674

coordinates relative to inaD-PA

CC,PBM domain
sufficient binding region
aa 863-1095

coordinates relative to norpA-PA

inaD 
PDZ domain residue 605
mutation decreasing interaction
aa 605

G605E, coordinates relative to inaD-PA

inaD 
PDZ domain residue 669
mutation decreasing interaction
aa 669

T669E, coordinates relative to inaD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected HEK293T cell extract.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)