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FB2025_05
,
released December 11, 2025
Factors were detected on polytene chromosomes using antibodies (to proteins) or biotinylated antisense probes (to ncRNA). The PLA signal develops only if probes to the two factors are in close proximity to each other (within tens of nanometers).
Source was larval salivary gland of wild-type fly line; proteins produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf21 cell system; prey produced as a recombinant fusion protein in baculovirus and Sf21 cell system.
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf21 cell system; prey produced as a recombinant fusion protein in baculovirus and Sf21 cell system.
Source was cell extract of Sf21 cell line; bait produced from infected baculovirus construct; prey produced from infected baculovirus construct.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell nuclear extract.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from Kc167 cell nuclear extract.
coordinates relative to Clamp-PB
Two-hybrid system: yeast GAL4-BD/GAL4-AD. Positive control.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
L139A; within the context of aa 1-153.
K146E; within the context of aa 1-153.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
L633A, V634A, E639A, Y634A; coordinates relative to msl-2-PA; within the context of aa 618-655.
L139A and K146E; within the context of aa 1-153.
K146E and R147E; within the context of aa 1-153.
Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Clamp residues with the most significant chemical shifts were located within the alpha-helix of the C2H2 zinc finger domain.
L139A and K146E; within the context of aa 40-153.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
msl-2 residues with the most significant chemical shifts were hydrophobic and charged clusters.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced as a recombinant fusion protein in baculoviral system.
Co-immunoprecipitation carried out in a chromatin assembly reaction lacking DNA (DREX extracts).
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced as a recombinant fusion protein in baculoviral system.
Co-immunoprecipitation carried out in a chromatin assembly reaction lacking DNA (DREX extracts).
Two-hybrid system: yeast GAL4-BD/GAL4-AD; positive control.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).