FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
abd-A, tin
FlyBase ID
FBig0000104277
Interaction Network
Interactions Browser links
abd-A network
tin network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0228100-10.coIP.WB
Reference
Baëza et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
abd-A 

AbdA

bait

HA tag

tin 

Tin

prey

Venus tag, N-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Anti-GFP antibody recognizing the Venus tag, N-terminal fragment used for western blot.

(Baëza et al., 2015)

Source was S2 cells; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Baëza et al., 2015)
FBrf0228100-22.FC.FM
Reference
Baëza et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
abd-A 

AbdA

bait

Venus tag, C-terminal fragment

abd-A 
abd-A 
abd-A 
abd-A 
tin 

Tin

prey

Venus tag, N-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
abd-A 
UbdA domain
mutation increasing interaction

KEINEQ/KAAAAA

abd-A 
HX domain
mutation increasing interaction

YPWM/YAAA

abd-A 
homeodomain residue
mutation disrupting interaction
aa 51

N51A abolishes the DNA-binding activity of abd-A and also inhibits this interaction.

abd-A 
homeodomain
sufficient binding region

Only the homeodomain fragment of abd-A is expressed.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in embryo; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Baëza et al., 2015)

To visualize the interaction, both proteins were tagged with a complementary fragment of the Venus fluorophore. Proximity of the two proteins resulted in reconstitution of the fluorescent protein.

(Baëza et al., 2015)
FBrf0240351-68.BiFC.FM
Reference
Bischof et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
abd-A 

AbdA

bait

CFP or YFP tag, N-terminal fragment

tin 

Tin

prey

CFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; proteins produced from tagged transgenic constructs.

(Bischof et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)