FB2021_01, released Feb 18, 2021

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
abd-A, tsh
FlyBase ID
FBig0000104659
Interaction Network
Interactions Browser links
abd-A network
tsh network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0213066-6.BiFC.FM
Reference
Hudry et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tsh 

tsh

neutral component

Venus tag, N-terminal fragment

tsh 

tsh

neutral component

Venus tag, N-terminal fragment

tsh 

tsh

neutral component

Venus tag, N-terminal fragment

abd-A 

abd-A

neutral component

Venus tag, C-terminal fragment

abd-A 

abd-A

neutral component

Venus tag, C-terminal fragment

abd-A 

abd-A

neutral component

Venus tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Hudry et al., 2011)

Embryos were collected overnight and incubated at 4 degrees C for 28 hrs prior to BiFC detection.

(Hudry et al., 2011)
FBrf0228100-26.FC.FM
Reference
Baëza et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
abd-A 

AbdA

bait

Venus tag, C-terminal fragment

abd-A 
abd-A 
tsh 

Tsh

prey

Venus tag, N-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
abd-A 
UbdA domain
mutation decreasing interaction

KEINEQ/KAAAAA Decreases the strength of the interaction in the embryonic epidermis.

abd-A 
HX domain
mutation increasing interaction

YPWM/YAAA Increases the strength of the interaction in the embryonic epidermis and the mesoderm, but not the nervous system.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in embryo; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Baëza et al., 2015)

To visualize the interaction, both proteins were tagged with a complementary fragment of the Venus fluorophore. Proximity of the two proteins resulted in reconstitution of the fluorescent protein.

(Baëza et al., 2015)
FBrf0240351-54.BiFC.FM
Reference
Bischof et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
abd-A 

AbdA

bait

CFP tag, N-terminal fragment

tsh 

tsh

prey

CFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; proteins produced from tagged transgenic constructs.

(Bischof et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)