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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000104666
Interaction Network
Interactions Browser links
Nedd4 network
Amph network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0231307-1.PD.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dNedd4Lo
bait
GST tag
Amph 
Amph
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N-terminal region
sufficient binding region
aa 1-63
coordinates relative to Nedd4-PJ
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.
FBrf0231307-5.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dNedd4Lo
bait
FLAG tag
Amph 
dAmph
prey
HA tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
FBrf0231307-6.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dNedd4Lo
bait
GST tag
Amph 
dAmph
prey
His tag
Amph 
Amph 
Amph 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N-terminal region
sufficient binding region
aa 1-63
coordinates relative to Nedd4-PJ
Amph 
SH3 domain
sufficient binding region
523-602
coordinates relative to Amph-PA
Amph 
SH3 domain
necessary binding region
523-602
coordinates relative to Amph-PA
Amph 
SH3 domain residue W580
mutation disrupting interaction
580
W580A, coordinates relative to Amph-PA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)