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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000104678
Interaction Network
Interactions Browser links
mys network
shv network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0232359-1.AT.FM
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mys 

╬▓PS integrin receptor

bait
shv 

Shv

prey

GFP tag

shv 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shv 
RGD motif
mutation disrupting interaction
KND

"LNV" construct

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

S2 cells were incubated with conditioned medium containing GFP-tagged CG4164 and processed for immunocytochemistry without permeabilization.

When S2 cells are incubated in CG4164-conditioned medium, CG4164 and mys colocalize at the cell surface and Fak becomes phosphorylated (which suggests integrin signaling).

Interaction in vitro; bait was present on live S2 cells; prey was produced from conditioned medium of transfected S2 cell line.

FBrf0235120-1.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shv 

shv

bait

HA tag

mys 

mys

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0235120-2.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shv 

shv

bait

HA tag

shv 
mys 

mys

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shv 
interaction domain
mutation disrupting interaction
aa 253,255

K253L,D255V

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult fly extract.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)