Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000105003
Interaction Network
Interactions Browser links
stx network
Pc network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0232699-1.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stx 

stx

bait

GFP tag

Pc 

Pc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of CME-W1-Cl.8+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0232699-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stx 

stx

bait

GFP tag

Pc 

Pc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0232699-3.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pc 

Pc

bait

GST tag

stx 

Stx

prey

MBP tag

stx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
stx 
Pc-binding domain
sufficient binding region
aa 210-300
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0232699-4.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stx 

stx

bait

FLAG tag

Pc 

Pc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

CG32676 interacts with Pc but not with other components of the PRC1 or PRC2 complexes: ph-p, ph-d, Psc, Sce, esc, E(z) or HDAC1. Expression of CG32676 decreases levels of Pc protein.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)