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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000105396
Interaction Network
Interactions Browser links
mir-305 network
p53 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0225691-1.miR-mRNA.GFP
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-305

bait
p53 

Dp53

prey
p53 
p53 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
p53 
3-prime UTR
sufficient binding region
p53 
mir-305 target site
necessary binding region
AUACUCUGUACAAA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic reporter construct.

Overexpression of mir-305 suppresses p53 reporter construct expression, while p53 reporter expression in increased in mir-305 mutant clones. These effects are dependent on the prediced mir-305 target site.

GFP reporter assay.

FBrf0225691-7.miR-mRNA.LUC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

mir-305

bait
p53 

Dp53

prey
p53 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
p53 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

Despite predicted target sites in the p53 3\'UTR, mir-1015, mir-985, mir-iab-4, mir-10 and mir-190 did not affect activity of the p53 3\'UTR luciferase reporter.

Luciferase reporter assay.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)