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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000105721
Interaction Network
Interactions Browser links
Raf network
Ras85D network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0183834-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

D-Ras

bait

FLAG tag

Raf 

D-Raf

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RasV12
mutation increasing interaction

constitutively active mutant

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

Wild type Ras85D did not associate with Raf in this experiment.

FBrf0210411-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Raf 

DRaf

bait

fused to GAL4 DNA-binding domain

Raf 
Raf 
Raf 
Raf 

Ras1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain
sufficient binding region
aa 1-183
Raf 
interaction domain
sufficient binding region
aa 17-212
Raf 
RBD domain
necessary binding region
aa 118-212
Raf 
RBD domain residue 174
mutation disrupting interaction
aa 174

R174L

Raf 
CRN domain
mutation decreasing interaction
aa 17-77

R174L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0218615-7.PD
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Raf 

Raf

bait

GST tag

Raf 

Ras1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Raf 
Ras binding domain
sufficient binding region
aa 128-212

coordinates relative to Raf-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Raf prefers to bind to GTP-bound Ras85D.

Positive control.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)