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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000105827
Interaction Network
Interactions Browser links
mir-277 network
mbl network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0233920-1.miR-mRNA.PCR
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

miR-277

bait
mbl 

mbl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Transgenic constructs were driven in muscles using Mhc-GAL4.

Source was adult male muscles of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

Expression of mir-277 sponge increased mbl mRNA levels 14-fold.

FBrf0233920-4.miR-mRNA.LUC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

miR-277

bait
mbl 

mbl

prey
mbl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mbl 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Luciferase reporter assay.

Source was HeLa cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

FBrf0233920-6.miR-mRNA.IHC
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

miR-277

bait
mbl 

mbl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Transgenic constructs were driven in muscles using Mhc-GAL4.

Expression of mir-277 sponge increased mbl protein detected in the cytoplasm by immunohistochemistry.

Source was adult male muscles of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 0 )
References (1)