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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000106502
Interaction Network
Interactions Browser links
cic network
rl network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0194799-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cic 

Cic

bait

fused to LexA DNA-binding domain

cic 
cic 
rl 

Rolled

prey

fused to B42 activation domain

rl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cic 
MAPK docking site
sufficient binding region
aa 1050-1079

coordinates relative to cic-PA

cic 
MAPK docking site
necessary binding region
aa 1054-1064

coordinates relative to cic-PA

rl 
C terminal region
necessary binding region

Authors used a form of rl lacking the C-terminal 52 amino acids that should be unable to bind substrates.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD

FBrf0194799-2.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cic 

Cic

bait

GST tag

cic 
cic 
rl 

Rolled

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cic 
MAPK docking site
sufficient binding region
aa 942-1116

coordinates relative to cic-PA

cic 
MAPK docking site
necessary binding region
aa 1054-1072

coordinates relative to cic-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0229058-1.XL.CM.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cic 

Cic

neutral component

GST tag

cic 

Cic

neutral component

GST tag

cic 

Cic

neutral component

GST tag

rl 

Rolled

neutral component

His tag

rl 

Rolled

neutral component

His tag

rl 

Rolled

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

FBrf0229058-2.XL.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cic 

Cic

neutral component

GST tag

cic 

Cic

neutral component

GST tag

cic 
cic 

Cic

neutral component

GST tag

cic 
rl 

Rolled

neutral component

His tag

rl 
rl 

Rolled

neutral component

His tag

rl 

Rolled

neutral component

His tag

rl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cic 
cross-linked peptide
necessary binding region
DGQQFILAPTPAQLGR
rl 
cross-linked peptide
necessary binding region
DVYIVQCLMETDLYK
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

Purified proteins were photocross-linked to stabilize the complex, protease digested, then cross-linked peptides identified by mass spectrometry.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)