FB2025_05 , released December 11, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cdc45, Psf1
FlyBase ID
FBig0000107322
Interaction Network
Interactions Browser links
Cdc45 network
Psf1 network
Reported Interactions
FBrf0193013-18.coIP.WB
Reference
Moyer et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc45 

Cdc45

bait
Psf1 

Psf1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Moyer et al., 2006)
FBrf0193013-8.coIP.MS
Reference
Moyer et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 15blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic stage 2embryonic cycle 9late extended germ band stageembryonic stage 5embryonic stage 12embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc45 

Cdc45

bait
Psf1 

Psf1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Moyer et al., 2006)
FBrf0214314-52.CS.EM
Reference
Costa et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Psf1 

Psf1

neutral component
Psf1 

Psf1

neutral component
Psf1 

Psf1

neutral component
Cdc45 

Cdc45

neutral component
Cdc45 

Cdc45

neutral component
Cdc45 

Cdc45

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced in a baculovirus and insect cell system.

(Costa et al., 2011)

The CMG complex is captured as 55 pairwise direct or indirect interactions.

(Costa et al., 2011)
FBrf0228822-16.EM
Reference
Costa et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdc45 

CDC45L

neutral component
Cdc45 

CDC45L

neutral component
Cdc45 

CDC45L

neutral component
Psf1 

Psf1

neutral component
Psf1 

Psf1

neutral component
Psf1 

Psf1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced in baculovirus and insect cell system.

(Costa et al., 2014)

Paper reports structure of large complex. Interactions of neighboring proteins curated in pairwise fashion.

(Costa et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)