FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
EcR, Cdk8
FlyBase ID
FBig0000107666
Interaction Network
Interactions Browser links
EcR network
Cdk8 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0229176-2.coIP.WB
Reference
Xie et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / prepupal stage P1
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait
Cdk8 

CDK8

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was white prepupae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Xie et al., 2015)
FBrf0229176-5.Y2H
Reference
Xie et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait

fused to GAL4 DNA-binding domain

EcR 
Cdk8 

dCDK8

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
EcR 
AF1 domain
sufficient binding region
aa 1-227

coordinates relative to EcR-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Xie et al., 2015)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Xie et al., 2015)
FBrf0229176-8a.coIP.MS
Reference
Xie et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / prepupal stage P1
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait
Cdk8 

cdk8

prey

9 peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was white prepupae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Xie et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)