FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eya, Socs44A
FlyBase ID
FBig0000108173
Interaction Network
Interactions Browser links
eya network
Socs44A network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0232812-3.coIP.WB
Reference
Hoi et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
eya 

Eya

bait

FLAG tag

Socs44A 

Socs44A

prey

HA tag

Socs44A 
Socs44A 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Socs44A 
SH2 domain
necessary binding region
aa 189-262

ΔSH2, coordinates relative to Socs44A

Socs44A 
SH2 domain residue R214
mutation disrupting interaction
aa 214

R214K, coordinates relative to Socs44A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hoi et al., 2016)

eya coimmunoprecipitates Socs44A much more strongly in the presence or absence of co-transfected Abl (which phosphorylates eya).

(Hoi et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)