FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
EcR, Smr
FlyBase ID
FBig0000108276
Interaction Network
Interactions Browser links
EcR network
Smr network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0190568-1.Y2H
Reference
Wang et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcRB1

bait

fused to GAL4 DNA-binding domain

Smr 

SMRTER

prey

fused to GAL4 activation domain

Smr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Smr 
interaction domain
sufficient binding region
aa 3068-3506
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Wang et al., 2006)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Wang et al., 2006)
FBrf0229176-18a.coIP.MS
Reference
Xie et al., 2015
Description
physical association
Assay
Collection
Source/Stage
organism / prepupal stage P1
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait
Smr 

Smr

prey

1 peptide identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was white prepupae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Xie et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)