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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108292
Interaction Network
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Hrb98DE network
nos network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0233508-2.coIP.PCR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

HRP38

bait
nos 

Nanos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0233508-7.coIP.XL.TV
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

HRP38

bait

GFP tag

nos 

Nanos

prey

biotin label

nos 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nos 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced and labeled by in vitro transcription.

Biotin label was detected using HRP-linked reagent.

FBrf0233508-8.coIP.XL.TV
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

HRP38

bait

RFP tag

nos 

Nanos

prey

biotin label

nos 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nos 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Biotin label was detected using HRP-linked reagent.

Interaction in vitro; bait produced as a tagged protein in S2-DRSC cell lysate; prey produced and labeled by in vitro transcription.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)