FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108416
Interaction Network
Interactions Browser links
dlg1 network
scrib network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0146937-3.coIP.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

SCRIB

bait
dlg1 

DLG

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval body wall muscle of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0236511-2.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

scribble

bait

GFP tag

dlg1 

Dlg

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult brains of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0242510-3.PLA
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Scrib

neutral component

Scrib

neutral component

Scrib

neutral component
dlg1 

Dlg

neutral component
dlg1 

Dlg

neutral component
dlg1 

Dlg

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of wild-type fly line; proteins produced from endogenous genes.

FBrf0243496-4.PLA
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Scrib

neutral component

GFP tag

Scrib

neutral component

GFP tag

Scrib

neutral component

GFP tag

dlg1 

Dlg

neutral component
dlg1 

Dlg

neutral component
dlg1 

Dlg

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wing discs of transgenic fly line; proteins produced from endogenous gene or tagged transgenic construct.

FBrf0246382-1.CL.FM
Description
colocalization
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Scrib

bait

GFP tag

dlg1 

Dlg

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
leucine-rich repeats
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Detection of protein-protein interactions through light-induced clustering. The ability of multimeric complexes of bait protein generated in response to light exposure to recruit prey protein was taken as evidence of physical interaction.

Source was fixed ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0251571-1.PL.WB
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dlg1 

dlg

bait

APEX2 tag

Scrib

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (6)