FB2025_02 , released April 17, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ovo, Ubr3
FlyBase ID
FBig0000108669
Interaction Network
Interactions Browser links
ovo network
Ubr3 network
Reported Interactions
FBrf0229651-1.coIP.WB
Reference
Zanet et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ovo 

Svb

bait

V5 tag

Ubr3 

Ubr3

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zanet et al., 2015)

Cells treated with MG132 to stabilize ovo protein from proteasomal degradation.

(Zanet et al., 2015)
FBrf0229651-2.coIP.WB
Reference
Zanet et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ubr3 

Ubr3

bait

GFP tag

ovo 

Svb

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from transfected S2 cell extract; prey derived from transfected S2 cell extract.

(Zanet et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)