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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108682
Interaction Network
Interactions Browser links
Su(dx) network
pyd network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0212737-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pyd 

pyd

unspecified role
pyd 

pyd

unspecified role
pyd 

pyd

unspecified role

Su(dx)

unspecified role

Su(dx)

unspecified role

Su(dx)

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: DNA binding domain and activation domain unspecified.

FBrf0212737-3.CL.FM
Description
colocalization
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pyd 

pyd

bait

HA tag

pyd 
pyd 

Su(dx)

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pyd 
Ex6 domain
mutation decreasing interaction
aa 791-869

coordinates relative to pyd-PB

pyd 
Pro-rich domain
mutation decreasing interaction
aa 870-1367

coordinates relative to pyd-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from tagged transfected construct; prey produced from tagged transfected construct.

Bait protein localizes to cell cortex while prey is largely cytoplasmic. The ability of this bait to recruit prey protein to cell cortex when co-expressed was taken as evidence of direct physical interaction.

FBrf0212737-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Su(dx)

bait

V5 tag

pyd 

pyd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0212737-5.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Su(dx)

bait

GST tag

pyd 

pyd

prey

[35]S label

pyd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
WW domain
sufficient binding region
unspecified
pyd 
C-terminal domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0212737-6.FS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Su(dx)

bait
pyd 

pyd

prey
pyd 
pyd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
WW domain
sufficient binding region
unspecified
pyd 
Ex6 domain
sufficient binding region
aa 861-870
pyd 
Pro-rich domain
sufficient binding region
aa 1287-1295
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as synthetic peptides.

pyd Ex6 peptide binds to WW2, WW1, WW1-2, WW3-4 domains of Su(dx) with decreasing affinity. pyd Pro-rich peptide binds only WW1-2 domain of Su(dx).

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)