FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
scrib, gukh
FlyBase ID
FBig0000108683
Interaction Network
Interactions Browser links
scrib network
gukh network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0146937-4.coIP.WB
Reference
Mathew et al., 2002
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval hypodermal muscle cell / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
scrib 

SCRIB

bait
gukh 

GUKH

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval body wall muscle of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Mathew et al., 2002)
FBrf0238421-1.ITC
Reference
Caria et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gukh 

Gukh

bait
gukh 
scrib 

Scrib

prey
scrib 
scrib 
scrib 
scrib 
scrib 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gukh 
C-terminal domain
sufficient binding region
aa 1781-1788

coordinates relative to gukh-PA

scrib 
PDZ1 domain
sufficient binding region
aa 726-820

coordinates relative to scrib-PD, Kd=664nM

scrib 
PDZ3 domain
sufficient binding region
aa 1237-1328

coordinates relative to scrib-PD, Kd=27.8uM

scrib 
PDZ1 domain residue 765
mutation decreasing interaction
aa 765

R765A, coordinates relative to scrib-PD, Kd=5.1uM

scrib 
PDZ1 domain residue 796
mutation disrupting interaction
aa 796

H796A, coordinates relative to scrib-PD

scrib 
PDZ1 domain residue 747
mutation disrupting interaction
aa 747

G747W, coordinates relative to scrib-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide commercially synthesized; prey produced as a recombinant fusion protein in bacterial system.

(Caria et al., 2018)
FBrf0238421-2.XC
Reference
Caria et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gukh 

Gukh

neutral component
gukh 

Gukh

neutral component
gukh 
gukh 

Gukh

neutral component
gukh 
scrib 

Scrib

neutral component
scrib 
scrib 

Scrib

neutral component
scrib 

Scrib

neutral component
scrib 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gukh 
C-terminal domain
sufficient binding region
aa 1781-1788

coordinates relative to gukh-PA

scrib 
PDZ1 domain
sufficient binding region
aa 726-820

coordinates relative to scrib-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; gukh peptide commercially synthesized; scrib produced as a recombinant fusion protein in bacterial system.

(Caria et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)