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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108713
Interaction Network
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inaD network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0103542-11.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait

Myc tag

inaD 

INAD

prey

FLAG tag

inaD 
inaD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
inaD 
PDZ3 domain
sufficient binding region
aa 346-457

coordinates relative to inaD-PA

inaD 
PDZ4 domain
sufficient binding region
aa 476-581

coordinates relative to inaD-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

Either PDZ3 or PDZ4 is sufficient for inaD homodimerization.

FBrf0103542-12.PD.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
inaD 

INAD

bait

GST tag

inaD 

INAD

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

External Crossreferences and Linkouts ( 0 )
References (1)