Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

ftz and ftz-f1 bind DNA cooperatively.

Various combinations of ftz and ftz-f1 were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast ftz-BD/GAL4-AD

This modified two hybrid system used full length ftz and a ftz proximal enhancer.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Deletion of the ftz LXXLL motif strongly reduces binding to ftz-f1, while deletion of part of the homeodomain has a more modest effect; however, deletion of both regions abolishes the ftz-ftz-f1 interaction.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Multiple regions of ftz-f1 interact with ftz.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

ftz and ftz-f1 bind to DNA cooperatively.

ftz and/or ftz-f1 were mixed with [32]P labeled DNA. DNA-protein complexes observed by EMSA were used to deduce protein-protein interactions.

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: mammalian GAL4-BD/VP16-AD