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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108898
Interaction Network
Interactions Browser links
4E-T network
eIF4E1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0226072-11.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
4E-T 

4E-T

bait

MBP tag

4E-T 

eIF4E

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
sufficient binding region
aa 69-248

coordinates relative to eIF4E1-PC

4E-T 
eIF4E-binding region
sufficient binding region
aa 9-44

coordinates relative to 4E-T-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0226072-16.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4E

bait

His tag

4E-T 

4E-T

prey

GB1 tag

4E-T 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
C-terminal region
sufficient binding region
aa 69-248

coordinates relative to eIF4E1-PC

4E-T 
eIF4E-binding region
sufficient binding region
aa 9-44

coordinates relative to 4E-T-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Kd = 5.6 nM

FBrf0226072-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4E

bait

HA tag

4E-T 

4E-T

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dorsal binding surface residue W106
mutation disrupting interaction
aa 106

W106A, coordinates relative to eIF4E1-PC

lateral binding surface residues I96 and I112
mutation disrupting interaction
aa 96-112

"II-AA" construct (I96A + I112A), coordinates relative to eIF4E1-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Mutation of residues on the lateral surface of eIF4E1 (I96A and I112A) specifically disrupts interaction with 4E-BP-type proteins (Thor, cup and 4E-T) but does not affect binding to eIF4G1.

FBrf0226072-8.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
4E-T 

4E-T

bait

HA tag

4E-T 
4E-T 
4E-T 
4E-T 
4E-T 

eIF4E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
4E-T 
N-terminal region
sufficient binding region
aa 1-511

coordinates relative to 4E-T-PB

4E-T 
canonical 4E-binding motif residues Y10 and L15
mutation disrupting interaction
aa 10-15

"C*" construct (Y10A + L15A), coordinates relative to 4E-T-PB

4E-T 
non-canonical 4E-binding motif
necessary binding region
aa 28-43

"ΔNC" construct, coordinates relative to 4E-T-PB

4E-T 
non-canonical 4E-binding motif residues L36 and L39
mutation disrupting interaction
aa 36-39

"NC*" construct (L36A + L39A), coordinates relative to 4E-T-PB

4E-T 
non-canonical 4E-binding motif residues F41-K43
mutation disrupting interaction
aa 41-43

"FWK-3xA" construct (F41A + W42A + K43A), coordinates relative to 4E-T-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

FBrf0243046-14.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

eIF4E

bait

HA tag

4E-T 

4E-T

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)