FB2022_03, released June 9, 2022

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
AGO1, mir-34
FlyBase ID
FBig0000109116
Interaction Network
Interactions Browser links
AGO1 network
mir-34 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204314-3.coIP.NB
Reference
Okamura et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait
mir-34 

miR-34

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Okamura et al., 2008)

AGO1 binds both the miRNA and miRNA* species.

(Okamura et al., 2008)
FBrf0223644-6.coIP.NB
Reference
Abe et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait
mir-34 

mir-34

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult male flies; bait produced from endogenous gene; prey produced from endogenous gene.

(Abe et al., 2014)
FBrf0242088-2.coIP.A
Reference
Goh and Okamura, 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
AGO1 

AGO1

bait
mir-34 

mir-34

prey

P[32] label

mir-34 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mir-34 
loop
sufficient binding region
nt 41-67

coordinates relative to mir-34-RM

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

AGO1 binds mir-34 more strongly than AGO2.

(Goh and Okamura, 2019)

Interaction in vitro; bait produced from S2R+ cell lysate; prey RNA oligo sythesized in vitro.

(Goh and Okamura, 2019)
External Crossreferences and Linkouts ( 0 )
References (3)