FB2025_02 , released April 17, 2025
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109384
Interaction Network
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Rok network
Shrm network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0211136-1.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rok 

dRok

bait

His tag

Rok 
Shrm 

dShrm

prey
Shrm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rok 
interaction domain
sufficient binding region
aa 821-938

coordinates relative to Rok-PA

Shrm 
SD2 domain
sufficient binding region
unspecified

SD2 domain included in aa 1442-1576, coordinates relative to Shroom-PA, but exact coordinates of fragment used not specified.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0219641-4.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shrm 

Shroom

bait

His tag

Shrm 
Rok 

Rok

prey
Rok 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shrm 
dimerization domain
sufficient binding region
aa 1393-1576

coordinates relative to Shroom-PD

Rok 
interaction domain
sufficient binding region
aa 724-948
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced in a bacterial expression system.

FBrf0219641-5.CM.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shrm 

Shroom

neutral component

His tag

Shrm 

Shroom

neutral component

His tag

Shrm 
Shrm 
Shrm 
Shrm 

Shroom

neutral component

His tag

Shrm 
Shrm 
Shrm 
Rok 

Rok

neutral component

His tag

Rok 
Rok 

Rok

neutral component

His tag

Rok 

Rok

neutral component

His tag

Rok 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shrm 
dimerization domain
sufficient binding region
aa 1393-1576

coordinates relative to Shroom-PD

Shrm 
dimerization domain
mutation decreasing interaction
aa 1468-1471aa 1546-1553

LLSL to AASA, coordinates relative to Shroom-PD

LIADARDL to AAADARDA, coordinates relative to Shroom-PD

Shrm 
SD2 domain
mutation decreasing interaction
aa 1470-1476aa 1509-1516

SLSERLA to ALEEDLE, coordinates relative to Shroom-PD

LKSDIERR to AASDIEDA, coordinates relative to Shroom-PD

Rok 
interaction domain
sufficient binding region
aa 724-948
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

FBrf0219641-6.PD.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rok 

Rok

bait

His tag

Shrm 

Shroom

prey
Shrm 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shrm 
SD2 domain
mutation decreasing interaction
aa 1470-1476aa 1509-1516

SLSERLA to ALEEDLE, coordinates relative to Shroom-PD

LKSDIERR to AASDIEDA, coordinates relative to Shroom-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced in a bacterial expression system.

FBrf0219641-7.FRET.F
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shrm 

Shroom

fluorescence donor

Cy3 tag

Shrm 

Shroom

fluorescence donor

Cy3 tag

Shrm 

Shroom

fluorescence donor

Cy3 tag

Rok 

Rok

fluorescence acceptor

Cy5 tag

Rok 

Rok

fluorescence acceptor

Cy5 tag

Rok 

Rok

fluorescence acceptor

Cy5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; fluorescence donor produced as a recombinant fusion protein in bacterial system; fluorescence acceptor produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)