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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109462
Interaction Network
Interactions Browser links
Ubx network
dsh network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0226424-9.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ubx 

Ubx

bait

fused to lexA DNA-binding domain

Ubx 
Ubx 
Ubx 
Ubx 
Ubx 
dsh 

dsh

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ubx 
interaction domain
sufficient binding region
aa 1-291

coordinates refer to Ubx-PA

Ubx 
interaction domain
necessary binding region
aa 1-215

coordinates refer to Ubx-PA

Ubx 
interaction domain
mutation decreasing interaction
aa 1-103

coordinates refer to Ubx-PA

Ubx 
interaction domain
mutation disrupting interaction
aa 1-103,250-292aa 1-103,292-389aa 103-216,250-292

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

coordinates refer to Ubx-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Transcriptionally inactive mutant A226P,Q233P of Ubx used.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)