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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000111423
Interaction Network
Interactions Browser links
Atg1 network
Unc-76 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0206363-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

UNC-76

bait

HA tag

Atg1 

UNC-51

prey

MYC tag

Atg1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N terminal domain
sufficient binding region
aa 1-200

coordinates relative to Unc-76-PA

N terminal domain
necessary binding region
aa 164-200

coordinates relative to Unc-76-PA

C terminal coiled coil domain
sufficient binding region
aa 277-395

coordinates relative to Unc-76-PA

Atg1 
C terminal domain
sufficient binding region
aa 508-855
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0206363-2.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

UNC-76

bait

GST tag

Atg1 

UNC-51

prey

MYC tag

Atg1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Atg1 
C terminal domain
sufficient binding region
aa 557-855

coordinates relative to Unc-51-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0206363-4.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Atg1 

UNC-51

enzyme

HA tag

Atg1 

UNC-51

enzyme

HA tag

Atg1 

UNC-76

enzyme target

His tag, P[32] label

UNC-76

enzyme target

His tag, P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)