FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-310, Khc-73
FlyBase ID
FBig0000111811
Interaction Network
Interactions Browser links
mir-310 network
Khc-73 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0212495-1a.miR-mRNA.LUC
Reference
Tsurudome et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mir-310 

mir-310

bait
Khc-73 

Khc-73

prey
Khc-73 
Khc-73 
Khc-73 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Khc-73 
3-prime UTR
sufficient binding region
Khc-73 
exon 14
sufficient binding region
Khc-73 
mir-310 conserved target sites
necessary binding region
UGCAAUA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Tsurudome et al., 2010)

Luciferase reporter assay.

(Tsurudome et al., 2010)
FBrf0212495-5a.miR-mRNA.WB
Reference
Tsurudome et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-310 

mir-310

bait
Khc-73 

Khc-73

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larvae of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Tsurudome et al., 2010)

Mutation of mir-310, mir-311, mir-312 and mir-313 by KT40 deletion increases levels of Khc-73 protein.

(Tsurudome et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)