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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000112100
Interaction Network
Interactions Browser links
CycA network
bam network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0235835-13.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bam 
Bam
bait
FLAG tag
CycA 
CycA
prey
Myc tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
FBrf0235835-14.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycA 
CycA
bait
FLAG tag
CycA 
CycA 
bam 
Bam
prey
Myc tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CycA 
C-terminal domain
sufficient binding region
aa 245-491
coordinates relative to CycA-PA
CycA 
C-terminal domain
necessary binding region
aa 245-491
coordinates relative to CycA-PA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
FBrf0235835-15.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bam 
Bam
bait
GFP tag
CycA 
CycA
prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
FBrf0235835-19.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycA 
CycA
bait
FLAG tag
bam 
Bam
prey
HA tag
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
CycA, bam and otu form a complex, captured as pairwise interactions.
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)