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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000112680
Interaction Network
Interactions Browser links
Raf network
Rap1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0183834-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rap1 

D-Rap1

bait

FLAG tag

Rap1 
Raf 

D-Raf

prey

MYC tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rap1 
RapV12
mutation increasing interaction

constitutively active mutant form of Rap1

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0210411-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Raf 

DRaf

bait

fused to GAL4 DNA-binding domain

Raf 
Raf 
Raf 
Raf 
Rap1 

Rap1

prey

fused to GAL4 activation domain

Rap1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rap1 
interaction domain
sufficient binding region
aa 1-180
Raf 
interaction domain
sufficient binding region
aa 17-212
Raf 
RBD domain
necessary binding region
aa 118-212
Raf 
RBD domain residue 174
mutation disrupting interaction
aa 174

R174L

Raf 
CRN domain
mutation decreasing interaction
aa 17-77

R174L

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)