Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast LexA-BD/B42-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 cell system; prey produced and labeled by in vitro translation.
