FB2020_06, released Dec 22, 2020

Physical Interaction report

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General Information
Interaction Type
physical association
Interacting Genes
shn, gro
FlyBase ID
FBig0000116023
Interaction Network
Interactions Browser links
shn network
gro network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0207974-3.coIP.WB
Reference
Cai and Laughon, 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shn 

Shn

bait

FLAG tag

shn 
shn 
gro 

Gro

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shn 
RD5 domain
sufficient binding region
aa 1133-1255

coordinates relative to a 2527aa isoform

shn 
RD5 domain
mutation decreasing interaction
aa 1201,1202,1203,1204

P1201A,L1202A,L1203A,G1204A, coordinates mapped to shn-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cai and Laughon, 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)