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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000116616
Interaction Network
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Hsp22 network
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0235730-1.DLS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DmHsp22

neutral component

DmHsp22

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Behavior observed is consistent with oligomeric structure of Hsp22 in solution. Hsp22 mutant, R110G, forms smaller oligomers.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

FBrf0235730-2.CH.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DmHsp22

neutral component

DmHsp22

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Wild-type Hsp22 eluted as a single peak of 790kD, mutant R109G had an estimated mass of 763kD and mutant R110G had an estimated mass of 680kD. The estimated mass of double mutant R109G,R110G was 369kD or 424kD depending on concentration.

FBrf0235730-3.CM.MW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

DmHsp22

neutral component

DmHsp22

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

Wild-type Hsp22 runs as a single band at 720kD. Hsp22 mutants, R109G and R105G, run as single bands slightly smaller than wild-type. Hsp22 mutants, R110G and R109GR11G, do not form a single band on a native gel.

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References (1)