FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000116634
Interaction Network
Interactions Browser links
Cpsf6 network
Atg1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0238799-7.coIP.PCR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CPSF6

bait

GFP tag

Atg1 

Atg1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RS domain
necessary binding region
aa 401-652

coordinates relative to CG7185-PA

RS domain
mutation disrupting interaction
aa 401-652

a series of 14 S or T to A mutations in the RS domain, residues 404,459,468, 525, 527, 531, 541, 543, 549, 571, 573, 588, 596, 622, coordinates relative to CG7185-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)